Advantages of a Clydes Herg Patch Clamp

A clydes herg patch clamp is used to record the electrical activity of heart cells. This technique is commonly used in pharmacology to screen drugs for potential cardiovascular effects. It uses transfected cell lines that contain single ion channels. Because the cells are isolated, they are more sensitive to ion-channel blockers. This system is suitable for many applications, including drug discovery. A few advantages of this method are listed below.



The current-tract recording was performed using a pClamp software package. The patch-clamp experiments were performed at room temperature. Data were acquired at a rate of 5 kHz and sampled at 10 kHz. Microcal Origin 7.0 was used for preliminary analysis and sigmoidal curves were fit with the Hill equation. The current-traces were filtered and sampled at a rate of 10 kHz.



The hERG-based method of drug herg patch clamp has become standard in pharmaceutical companies. It has several applications including the assessment of potential proarrhythmic risks associated with a drug. It can also be used to screen multiple ion channels such as Nav1.5 and Cav1.2. The patch-clamp method can be automated and can simultaneously assess up to 48 different drugs and 128 different kinds of candidate drugs. Despite its limitations, it has become a routine in the pharmaceutical industry.

The instrument is easy to use. Harvested cells and compound plates are loaded into the instrument. The instrument has specific settings for each cell type. It has voltage protocols for eliciting ion channel currents. The application protocol defines the order in which the drugs are applied to the cells. The instrument manufacturer will usually supply settings for common hERG-expressing cell lines. The following sections describe more details. They are available on the manufacturer's website.



The quality of the cells is highly dependent on the incubation time for the cells in the cell detachment agent. If the time is too short, they may interact with mechanically, resulting in poor success rates. In addition, the amount of cell damage is determined by trypan blue staining, which should be at least 5%. While an incubation period of more than 24 hours is usually sufficient to obtain an excellent seal rate, the cells may not be stable enough afterward.

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